1601547

12073

Relative reactivities of proteins to radical reactions caused in human plasma were studied for the first time utilizing an immunoblotting assay. When radical reactions were caused by Cu2+, apolipoprotein B-100 (apoB) underwent extensive fragmentation concurrently with the decrease in α-tocopherol, while human serum albumin (HSA) and transferrin (TF) were not decreased at all. When radical reactions were initiated by Cu2+ with hydrogen peroxide or 2,2′-azobis(2-amidinopropane)dihydrochloride (AAPH), α-tocopherol and apoB were also decreased steadily but HSA and TF were not decreased. These observations indicate that apoB is extremely reactive, even comparable to α-tocopherol, towards radical reactions. These results also suggest that the radical reaction of apoB is a possible process in vivo and it is involved in atherogenesis along with low density lipoprotein lipid peroxidation, which has been studied extensively.

236

transferrin , Albumin , apolipoprotein B-100 , low density lipoprotein , protein degradation , Radical

Studia Iranica

240

240