Determination of the association between the C677T and A1298C polymorphisms of the MTHFR gene and the development risk of azoospermia and oligozoospermia in Turkish infertile men

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Aim: We aimed to investigate the relationship between unexplained male infertility, and the -677C/T (rs1801133) and -1298A/C (rs1801131) polymorphisms of the MTHFR gene in a group of Turkish infertile men with non-obstructive azoospermia and severe oligozoospermia in this study.Materials and Methods: Study group includes 50 non-obstructive azoospermic patients, 50 severe oligospermic patients and 50 healthy controls with normal sperm parameters who had had more than one child. Genotyping was performed by generated amplicons from melting curve analysis after real time PCR.Results: The distribution of the 677CC genotype was significantly higher in the control group than the infertile group (p= 0.046). There was a significant frequency of the polymorphic T allele in infertile patients higher than the control group (p=0.015). Neither the frequency, nor the allelic distribution of A1298C genotype was different between infertile groups compared with the control.Conclusions: The MTHFR 677TT genotype is a genetic risk factor for unexplained male infertility, especially in the group with oligospermia and non-obstructive azoospermia

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MTHFR , C677T , A1298C , polymorphism , azoospermia , oligospermia

Ege Journal Of Medicine

128

Ege Journal Of Medicine