Author/Authors
kaplan, selçuk namık kemal university - faculty of veterinary medicine - department of genetics, Turkey
Title Of Article
Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method
شماره ركورد
33707
Abstract
Leptin is a hormone which produces 16 kDa protein predominantly expressed in adipose tissue. Leptin gene has pleiotropic effects on several functional traits such as milk yield, carcass and meat quality, growth, feed intake and fertility. Therefore, this study was performed to identify single nucleotide polymorphism of bubaline leptin gene in Anatolian buffaloes. In this study, 513 bp of leptin DNA fragment was amplified. A PCR-RFLP analysis was used to identify bubaline leptin gene T1131G polymorphism. DdeI restriction enzyme was used to genotype bubaline leptin gene T1131G polymorphism in Anatolian buffaloes. The analysis revealed TT, GT and GG genotypes in Anatolian buffaloes. Allele frequencies of T and G were found 0.478 and 0.521, respectively. This study is the first report of identification of bubaline leptin gene T1131G polymorphism by using PCR-RFLP method in Anatolian buffaloes.
From Page
93
NaturalLanguageKeyword
Leptin , Anatolian Buffalo , SNP , PCR , RFLP
JournalTitle
Alınteri Journal Of Agricultural Science
To Page
97
JournalTitle
Alınteri Journal Of Agricultural Science
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