Author/Authors
NUR, Zekariya Uludag University - Veterinary Faculty - Department of Reproduction and Artificial Insemination, Turkey , ZIK, Berrin Uludag University - Veterinary Faculty - Department of Reproduction and Artificial Inseminationı, Turkey , USTUNER, Burcu Uludag University - Veterinary Faculty - Department of Reproduction and Artificial Insemination, Turkey , TUTUNCU, Serife Uludag University - Veterinary Faculty - Department of Histology and Embryology, Turkey , SAGIRKAYA, Hakan Uludag University - Veterinary Faculty - Department of Reproduction and Artificial Insemination, Turkey , OZGUDEN, Cansel G. Uludag University - Veterinary Faculty - Department of Histology and Embryology, Turkey , GUNAY, Ulgen Uludag University - Veterinary Faculty - Department of Reproduction and Artificial Insemination, Turkey , DOGAN, Ibrahim Uludag University - Veterinary Faculty - Department of Reproduction and Artificial Insemination, Turkey
Title Of Article
Effect of freezing rate on acrosome and chromatin integrity in ram semen
شماره ركورد
34926
Abstract
The objective of the present study was to investigate the effect of different freezing rates on post-thaw sperm motility, acrosome defect, and sperm chromatin structure and apoptotic activity in ram semen. Collected semen was diluted at 1:5 (semen/extender) with Bioxel® (IMV technologies France) at 30ºC and then cooled to 5ºC within 1h. Cooled semen was subjected to the equilibration for 2 hours. Equilibrated semen was frozen in 0.25 ml straw at two different cooling rates (slow: 0.5°C/min from 5 to -20°C and fast: 5°C/min from 5 to -20°C). Both groups were frozen from -20 to -120°C at 25°C/min and stored in liquid nitrogen until use. Post-thaw (37°C/30 min) sperm motility, defected acrosome (Pisum sativum agglutinin fluorescein conjugate, FITC PSA), sperm chromatin structure determined by Acridin Orange (AO) and apoptotic activity using terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL) were evaluated. Post-thaw sperm motility, acrosome defect, AO and TUNEL for slow frozen semen were 42.8±8.8%, 31.6±12.9%, 2.9±2.4% and 2.8±1.6%, and for fast frozen semen were 36.5±9.9%, 24.7±11.1%, 3.3±2.2% and 6.3±3.4%, respectively. Post-thaw semen analyses showed that there was no significant difference between two freezing curves in terms of acrosome defect, sperm chromatin damage (AO). However, a significant difference was found for postthaw semen motility between two groups (P 0.05). In conclusion, while the slow freezing procedure improved post-thaw sperm motility, acros
From Page
267
NaturalLanguageKeyword
Apoptosis , freezing rate , ram semen
JournalTitle
Veterinary Journal of Ankara University
To Page
272
JournalTitle
Veterinary Journal of Ankara University
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