DECOLORIZATION OF DENIM DYESTUFF BY LACCASE ENZYME

41954

Large quantities of dyes used in the textile industry are discharged to recipient environment during manufacture. This situation is beginning of a process which is difficult to recovery and relevant to environment and human health. Therefore, pollution of dyestuff produced textile industry will be reduced by cleaning of polluted area and integrating biological approaches with technologies having polluting potential. In scope of this study, commercial denim dye was decolorized by using high laccase activity culture supernatant of Trametes versicolor ATCC 200801 pellets grown in potato dex-trose broth including wheat bran and determined optimum conditions. In the result of experiments done, pH, initial dye concentration, temperature and incubation time were selected 4.0, 75 mg/l, 55 °C and 120 minutes, respectively. 68.02 % of decolorization was obtained at the determined optimum conditions. Furthermore, adding different metal ions to find in textile wastewater and supplementary chemical materials used fabric dyeing process to reaction medium, potential of decolorization copiedwith improvement was investigated effects of these. When the obtained data were examined, pollutants which tested at optimum conditions were observed not affected negatively decolorization. Even in the presence of Tween 80 detected the maximum inhibitor effect, 54.68 % of decolorization was obtained.

59

Laccase , Dyestuff , Decolorization

Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology

70

Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology