Author/Authors
ÜNAL, Arzu Ministry of Agriculture and Rural Affairs - General Directorate of Agricultural Research, TURKEY , ÇABUK, Ahmet Eskişehir Osmangazi University - Science and Arts Faculty - Department of Biology, TURKEY , KOLANKAYA, Nazif Hacettepe University, Beytepe Campus - Science Faculty - Department of Biology, Division of Biotechnology, TURKEY
Title Of Article
DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR
شماره ركورد
41960
Abstract
Detoxification of a chlorinated phenolic compound, 2,4,6-trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymatic dechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuously to detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium.The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6-trichlorophenol by the laccase under the determined optimum conditions.
From Page
113
NaturalLanguageKeyword
Laccase , Trametes versicolor , 2 , 4 , 6 , tricholorophenol , Dechlorination
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
To Page
124
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
Link To Document