DETECTION OF CRYPTOSPORIDIUM OOCYSTS BY LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN SURFACE WATER FROM RIVER YEŞİLIRMAK AND STREAM TERSAKAN (SAMSUN-AMASYA)

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The water samples collected from six stations of Stream Tersakan which include Havza; Suluova-Celtek, Kanlıdere, Bogazkoy, Karasu, Tersakan-Last and eight stations of River Yesilirmak which include Amasya Bridge, Yassıcal, Durucasu, Tasova, Kumkoy, Carsamba entrance, Carsamba Bridge, Hacıomer in Amasya and Samsun Provinces were investigated for detection of Cryptosporidium oocysts by Loop Mediated Isothermal Amplification (LAMP). The water samples were regularly collected in every month from the 14 stations in investigated area between 2010 December to 2011 November for LAMP tests.Ten of 14 investigated stations were found contaminated with Cryptosporidium oocyst by LAMP. The pollution with Cryptosporidium oocysts were determined in stations which are Tersakan–Havza; Tersakan-Suluova, Celtek; Tersakan-Kanlıdere; Tersakan-Bogazkoy; Tersakan-Karasu Tersakan-Last, Yesilirmak-Amasya Bridge, Yesilirmak-Yassıcal, Yesilirmak-Durucasu, Yesilirmak-Tasova. The high contamination rate of Cryptosporidium oocysts were found in Tersakan–Suluova, Celtek and Tersakan-Bogazkoy stations in the all investigated stations. However, River Yesilirmak which is last in Samsun more clean than Stream Tersakan which is last in Amasya in terms of the Cryptosporidium oocysts contamination. The reason in excess of the rate of Cryptosporidium contamination in Stream Tersakan are more animal husbandry in Havza and Suluova Boroughs and no processing domestic and agricultural waste water mixing to surface water.

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Amasya , Cryptosporidium , LAMP , Samsun , Surface waters

Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology

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Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology