ASSESSING THE DIVERSITY OF PROKARYOTIC COMMUNITIES AND NANOHALOARCHAEAL LINEAGES IN VARIOUS AQUATIC HYPERSALINE HABITATS (TURKEY) USING HIGH-THROUGHPUT SEQUENCING AND CLONING

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In this study, distribution of the prokaryotic groups in Tuz Lake, Ayvalık and Tuzlagözü solar salterns was investigated using 16S rRNA gene targeted approaches. The relative abundance of prokaryotic taxa in the samples was detected by using high-throughput sequencing. Operational taxonomic units (OTUs) associated with Haloquadratum were the most abundant in MiSeq reads. Nanohaloarchaeota-related OTUs were rare ( 1%) in Ayvalık and Tuzlagözü solar salterns, and around 5% in Tuz Lake. Other OTUs frequently found and shared in the samples were associated with Halorubrum, Halonotius and Salinibacter genera. Nanohaloarchaeota lineages, whose phylotypes have been frequently detected in diverse hypersaline environments, were examined in more detail by 16S rRNA gene cloning using group-specific primer. Some of the highly represented nanohaloarchaeal phylotypes in the clone libraries showed low similarity to any sequence in the database, generating two distinct clades. One of the novel lineages was found to be prominent in the clone library constructed from Ayvalık sample. Phylotypes showing 95-97% sequence similarity to Ca. Nanopetramus were also highly represented in Ayvalık. Phylotypes frequent in the clone libraries of Tuz Lake and Tuzlagözü samples were associated with a novel lineage, as well as Ca. Nanosalina and its relatives.

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Nanohaloarchaeota , High , Throughput Sequencing , Cloning , Halophiles

Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology

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Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology