Author/Authors
yayci, gülay sağlık bilimleri üniversitesi (sbü) - van education and research hospital, Van, turkey , dede, semiha van yuzuncu yil university - veterinary medical faculty - biochemistry department, Van, turkey , usta, ayse van yuzuncu yil university - science faculty - chemistry department, Van, turkey , yuksek, veysel van yuzuncu yil university - özalp vocational school, Van, turkey , çetin, sedat van yuzuncu yil university - veterinary medical faculty - biochemistry department, Van, turkey
Title Of Article
IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION
شماره ركورد
44707
Abstract
This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 μM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.
From Page
132
NaturalLanguageKeyword
High glucose , Glutathione , Apoptosis , DNA damage , in vitro , Kidney cells
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
To Page
137
JournalTitle
Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology
Link To Document