Magnetic Barcode Nanowires for Osteosarcoma Cell Control, Detection and Separation

Author

Sharma, Anirudh ; Zhu, Yuechen ; Thor, ShengShee ; Zhou, Fang ; Stadler, Bethanie ; Hubel, Allison

Author_Institution

Electr. & Comput. Eng. Dept., Univ. of Minnesota, Minneapolis, MN, USA

Volume

49

Issue

1

fYear

2013

fDate

Jan. 2013

Firstpage

453

Lastpage

456

Abstract

Multilayered gold/nickel “barcode” nanowires were grown using electrodeposition for multiplexed detection in biomolecular assays. Non-specific uptake of barcoded nanowires by osteosarcoma cells (OSCA-8) was observed by scanning and transmission electron microscopy images. Barcode-tagged cells were manipulated with an external magnetic field and were readily separated from untagged cells. Separation efficiency increased from a maximum of 30.14% to a maximum of 47.9% as clustering was reduced. Cells exposed to nanowires (both those that incorporated wires and those that did not) appeared to be viable suggesting that the nanowires are not toxic.

Keywords

biomagnetism; bone; cancer; cellular biophysics; electrodeposition; gold; magnetic multilayers; nanobiotechnology; nanofabrication; nanomagnetics; nanowires; nickel; scanning electron microscopy; toxicology; transmission electron microscopy; Au-Ni; biomolecular assay multiplexed detection; electrodeposition; multilayered gold-nickel barcode nanowires; osteosarcoma cell control; osteosarcoma cell detection; osteosarcoma cell separation; scanning electron microscopy images; toxicity; transmission electron microscopy image; Gold; Magnetic force microscopy; Magnetic resonance imaging; Magnetic separation; Microscopy; Nanowires; Nickel; Cell manipulation; cellular uptake; magnetic carriers; magnetic separation; nanowires;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/TMAG.2012.2224647

Filename

6392423