Microelectrode Array (MEA) Platform for Targeted Neuronal Transfection and Recording

Author

Jain, Tilak ; Muthuswamy, Jit

Volume

55

Issue

2

fYear

2008

Firstpage

827

Lastpage

832

Abstract

Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.

Keywords

biomedical electrodes; genetics; microelectrodes; molecular biophysics; neurophysiology; cathodic pulse; microelectrode array platform; microelectroporation; nonviral gene transfection; siRNA molecules; targeted neuronal recording; targeted neuronal transfection; transfection efficiency; Biomedical engineering; Circuits; DNA; Fabrication; Interference; Microelectrodes; Neurons; Neuroscience; RNA; Spatial resolution; Biochip; electroporation; siRNA; transfection; Animals; Cell Culture Techniques; Electroporation; Gene Therapy; Microelectrodes; Neurons; Protein Array Analysis; RNA, Small Interfering; Rats; Rats, Sprague-Dawley; Transfection;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2007.914403

Filename

4432741