A sensitive polarized fluorometer for clinical tests

Author

Lago, Paolo ; Rovati, Luigi ; Corti, Mario

Author_Institution

Dept. of Electron., Pavia Univ., Italy

Volume

43

Issue

4

fYear

1994

fDate

8/1/1994 12:00:00 AM

Firstpage

664

Lastpage

667

Abstract

A fully automated fluorometer which measures fluorescence depolarization is described. The instrument has been designed to work with standard microtiter sample holders normally used in clinical tests. The combination of a hybrid optical system, built with discrete optical elements and fiber bundles, and a digital Fourier analysis of the detected signals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10^-3 and a minimum content of fluorescein in the sample well of 20 pmol

Keywords

Fourier analysis; biomedical equipment; biomedical measurement; computerised spectroscopy; light polarisation; medical signal processing; optical fibres; automated fluorometer; clinical tests; computer control; digital Fourier analysis; discrete optical elements; fiber bundles; fluorescein; fluorescence depolarization; hybrid optical system; microtiter sample holders; polarization ratio; reproducibility; sensitive polarized fluorometer; signal analysis; Batteries; Buttocks; Instruments; Microcomputers; Orthotics; Polarization; Surface treatment; Testing; Transducers; Wire;

fLanguage

English

Journal_Title

Instrumentation and Measurement, IEEE Transactions on

Publisher

ieee

ISSN

0018-9456

Type

jour

DOI

10.1109/19.310186

Filename

310186