Polarized light scattering spectroscopy for quantitative measurement of epithelial cellular structures in situ

Author

Backman, Vadim ; Gurjar, Rajan ; Badizadegan, Kamran ; Itzkan, Irving ; Dasari, Ramachandra R. ; Perelman, Lev T. ; Feld, Michael S.

Author_Institution

G.R. Harrison Spectroscopy Lab., MIT, Cambridge, MA, USA

Volume

5

Issue

4

fYear

1999

Firstpage

1019

Lastpage

1026

Abstract

We report an in situ method of probing the structure of living epithelial cells, based on light scattering spectroscopy with polarized light. The method makes it possible to distinguish between single backscattering from uppermost epithelial cells and multiply scattered light. The spectrum of the single backscattering component can be further analyzed to provide histological information about the epithelial cells such as the size distribution of the cell nuclei and their refractive index. These are valuable quantities´ to detect and diagnose precancerous changes in human tissues

Keywords

backscatter; biological techniques; cancer; cellular biophysics; light polarisation; light scattering; patient diagnosis; refractive index; cell nuclei; epithelial cellular structures in situ; histological information; human tissues; living epithelial cells; multiply scattered light; polarized light scattering spectroscopy; precancerous changes; quantitative measurement; refractive index; single backscattering component; size distribution; uppermost epithelial cells; Backscatter; Biomedical measurements; Cancer; Cells (biology); Data mining; Light scattering; Microscopy; Optical polarization; Optical scattering; Spectroscopy;

fLanguage

English

Journal_Title

Selected Topics in Quantum Electronics, IEEE Journal of

Publisher

ieee

ISSN

1077-260X

Type

jour

DOI

10.1109/2944.796325

Filename

796325