• DocumentCode
    1305317
  • Title

    Summarizing Probe Intensities of Affymetrix GeneChip 3´ Expression Arrays Taking into Account Day-to-Day Variability

  • Author

    Magni, Paolo ; Simeone, Angela ; Healy, Sandra ; Isacchi, Antonella ; Bosotti, Roberta

  • Author_Institution
    Dipt. di Inf. e Sist., Univ. degli Studi di Pavia, Pavia, Italy
  • Volume
    8
  • Issue
    5
  • fYear
    2011
  • Firstpage
    1425
  • Lastpage
    1430
  • Abstract
    Microarray experiments are affected by several sources of variability. The paper demonstrates the major role of the day-to-day variability, it underlines the importance of a randomized block design when processing replicates over several days to avoid systematic biases and it proposes a simple algorithm that minimizes the day dependence.
  • Keywords
    genetics; lab-on-a-chip; probes; Affymetrix Genechip 3´ expression arrays; day dependence; day-to-day variability; microarray experiments; probe intensities; randomized block design; systematic biases; Algorithm design and analysis; Arrays; Bioinformatics; Correlation; Gene expression; Probes; Affymetrix microarray; Gene expression; experimental variability.; statistical analysis; Algorithms; Cell Line, Tumor; Cluster Analysis; Computational Biology; Gene Expression Profiling; Humans; Models, Statistical; Molecular Probes; Oligonucleotide Array Sequence Analysis; Reproducibility of Results;
  • fLanguage
    English
  • Journal_Title
    Computational Biology and Bioinformatics, IEEE/ACM Transactions on
  • Publisher
    ieee
  • ISSN
    1545-5963
  • Type

    jour

  • DOI
    10.1109/TCBB.2010.82
  • Filename
    5557856
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=1305317