Optical projection tomography and light sheet microscopy for imaging in biological specimens a comparison study

Author

Psycharakis, Stylianos ; Zacharopoulos, Athanasios ; Ripoll, Jorge ; Zacharakis, Giannis ; Riekher, Matthias ; Tavernarakis, Nektarios

Author_Institution

Inst. of Electron. Struct. & Laser, Found. for Res. & Technol.-Hellas, Heraklion, Greece

fYear

2014

fDate

14-17 Oct. 2014

Firstpage

211

Lastpage

215

Abstract

Optical Projection Tomography and Light Sheet Microscopy have been recent revolutionary techniques for imaging in biological specimens with 3D volume rendering of high resolution. A comparison study is performed on the basic aspects of each modality and combined application on selected specimen is presented. The imaging performance characteristics of the two modalities are calculated and analyzed. Studies in model organisms such as Caenorhabditis Elegans and Parhyale, are shown and the significance of each method is discussed in relation to the specific applications they are best tailored for.

Keywords

biomedical optical imaging; image resolution; microorganisms; optical tomography; rendering (computer graphics); 3D volume rendering; Caenorhabditis Elegans; Parhyale; biological specimen imaging; high-resolution images; imaging performance characteristics; light sheet microscopy; optical projection tomography; Biomedical optical imaging; Fluorescence; Microscopy; Optical imaging; Optical microscopy; Optimized production technology;

fLanguage

English

Publisher

ieee

Conference_Titel

Imaging Systems and Techniques (IST), 2014 IEEE International Conference on

Conference_Location

Santorini

Type

conf

DOI

10.1109/IST.2014.6958475

Filename

6958475