Individually addressable planar patch clamp array

Author

Cheung, Karen ; Kubow, Tim ; Lee, Luke P.

Author_Institution

Dept. of Bioeng., California Univ., Berkeley, CA, USA

fYear

2002

fDate

6/24/1905 12:00:00 AM

Firstpage

71

Lastpage

75

Abstract

We present a new planar, silicon-based patch clamp array. Each patch site is vertically oriented, which allows simultaneous use of scanning probe, optical, or fluorescent detection technologies. Each microfabricated patch site is also connected to its own fluidic channel so that the array can be used for parallel screening of cells or analysis of tethered lipid membranes or vesicles that express the proteins of interest. A single unit, fully three-dimensional ring-shaped patch clamp recording device has been designed and fabricated

Keywords

arrays; biocontrol; bioelectric potentials; biomembrane transport; biosensors; microelectrodes; microfluidics; microsensors; nozzles; photolithography; silicon; sputter etching; 3D ring-shaped recording device; Si; cell signalling; deep RIE; fluidic channel; fluorescent detection; glass micropipette; individually addressable array; ion channels; living cells; microfabricated patch site; optical detection; optimal pipette size; parallel screening of cells; photolithography; planar patch clamp array; ring-shaped nozzle; scanning probe detection; simultaneous use; tethered lipid membranes; transmembrane proteins; vertically oriented; vesicles; Biomedical electrodes; Biomedical engineering; Biomembranes; Cells (biology); Clamps; Glass; Neurons; Probes; Proteins; Seals;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on

Conference_Location

Madison, WI

Print_ISBN

0-7803-7480-0

Type

conf

DOI

10.1109/MMB.2002.1002267

Filename

1002267