Development of "IISA-ATP" system for in situ microbial activity assessment in deep-sea environment

Author

Aoki, Yusuke ; Fukuba, Tatsuhiro ; Yamamoto, Takatoki ; Fujii, Teruo

Author_Institution

Inst. of Ind. Sci., Tokyo Univ., Tokyo

fYear

2008

Firstpage

1

Lastpage

4

Abstract

In this study, "Integrated in situ analyzer-ATP (IISA-ATP)" which is to estimate microbial activities in deep-sea environments has been developed. On the system, a microfluidic device is used as a core element to perform a bioluminescent (luciferin-luciferase) reaction for ATP quantification. The microfluidic device has a coil-shaped microchannel for photo intensity measurement. In this paper, concept of the IISA-ATP and optimization process of the microchannel design are presented. As a result of the microchannel design and flow rate optimization, 2 pM to 2 x 10⁴ pM (20 nM) of ATP was successfully quantified.

Keywords

oceanographic equipment; IISA-ATP; Integrated in situ analyzer-ATP; bioluminescent reaction; deep-sea environments; flow rate optimization; luciferin-luciferase; microbial activities; microchannel design; microfluidic device; photo intensity measurement; Bioluminescence; Chemical analysis; Design optimization; Extracellular; Microchannel; Microfluidics; Microorganisms; Ocean temperature; Sea measurements; Spatial resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

OCEANS 2008 - MTS/IEEE Kobe Techno-Ocean

Conference_Location

Kobe

Print_ISBN

978-1-4244-2125-1

Electronic_ISBN

978-1-4244-2126-8

Type

conf

DOI

10.1109/OCEANSKOBE.2008.4530991

Filename

4530991