Evaporation-driven microfluidic sample concentration

Author

Walker, G.M. ; Beebe, D.J.

Author_Institution

Dept. of Biomed. Eng., Wisconsin Univ., Madison, WI, USA

fYear

2002

fDate

6/24/1905 12:00:00 AM

Firstpage

523

Lastpage

526

Abstract

A new method for sample concentration within microfluidic devices is presented. Evaporation at an air/liquid interface is used to induce flow. within a microfluidic device and concentrate sample. The practicality of this method was demonstrated with 0.2 μm fluorescent microspheres and FITC-labeled bovine serum albumin (BSA). Eighty one percent of a 0.6 μL fluorescent sphere suspension was concentrated into a well within a microfluidic device. In the same amount of time, 93% of a 0.6 μL FITC-labeled BSA solution was concentrated

Keywords

biological techniques; evaporation; mass transfer; microfluidics; separation; suspensions; air/liquid interface; average velocities; bovine serum albumin; concentration gradient; equivalent volumetric flow rates; evaporation-driven; fluorescent microspheres; mass transfer coefficient; microfluidic devices; polydimethylsiloxane mold; rapid prototyping; sample concentration; straight micro fluidic. channel; Biological system modeling; Biomedical engineering; Fluorescence; Microchannel; Microfluidics; Reservoirs; Solids; Stacking; Surface treatment; Water resources;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on

Conference_Location

Madison, WI

Print_ISBN

0-7803-7480-0

Type

conf

DOI

10.1109/MMB.2002.1002397

Filename

1002397