Protective effect of Xiongma pills-containing serum on SNP oxidative stress-injury in PC12 cells

Oxidative stress, active nitrogen, induced cell damage had been implicated in a variety of neurodegenerative disorders. To observe the protective effect and antioxidant mechanism of Xiongma pills-containing serum on active nitrogen, injury in PC12 cells, we established the model of oxidative stress by NO exhibitor, sodiumnitroprusside(SNP). Cell viability, lactate dehydrogenase release(LDH) in culture supernatants and glutathione peroxidase (GSH-PX) activities in suspension cells were determined by MTT assay and chemistry determination, respectively. SNP could induce PC12 cell damage and decrease survival rate of PC12 cells significantly, increase LDH leakage and decrease GSH-PX activities. Xiongma pills-containing serum which the administration dose was equivalent 0.75, 1.5, 3, 6.g·kg^-1 crude drug respectively could significantly increase the survival rate of PC12 cells and GSH-PX activities, and decrease LDH leakage. And Xiongma pills 6 g·kg-1 crude drug was the best dose. The result suggests that Xiongma pills-containing serum has a neuroprotective effect against SNP-induced antioxidant through mechanisms related to improve the activity of antioxidative enzymes via and scavenge reactivity oxygen species.