Capacitive monitoring of the antigen-antibody reactions enhanced by nanogold

Author

Guo, X.S. ; Fang, X.S. ; Yang, X.L. ; Chen, Y.Q. ; Wang, L.R.

Author_Institution

Dept. of Biomed. Eng., Zhejiang Univ., Hangzhou

fYear

2005

fDate

6/27/1905 12:00:00 AM

Firstpage

1260

Lastpage

1263

Abstract

An immunosensor for measuring antigen-antibody binding by nanogold particles enhancement was presented here. A gold plate electrode was modified by a SAM (self-assembled monolayer) of 11-mercaptoundecanioc acid. Then sheep anti-rabbit IgG was immobilized onto the end of the 11-mercaptoundecanioc acid. With that the gold electrode was exposed to the mixed solution of rabbit IgG and nanogold labeled sheep anti-rabbit IgG. The differential capacitance of the electrical double layer was monitored to detect the antigen-antibody reaction. The result showed the capacity of the electrical double layer by nanogold enhancement was largely increased compared to that in traditional capacitive immunosensor. The detection sensitivity is up to 10nF/mug/ml

Keywords

biochemistry; capacitive sensors; electrochemical electrodes; electrochemistry; gold; microsensors; monolayers; nanobiotechnology; nanoparticles; self-assembly; 11-Mercaptoundecanioc Acid; Au; SAM; antigen-antibody reactions; capacitive monitoring; electrical double layer; electrode; immunosensor; nanogold particles; self-assembled monolayer; sheep anti-rabbit IgG; Biomedical electrodes; Biomedical engineering; Biosensors; Capacitance; Gold; Immune system; Monitoring; Nanobioscience; Rabbits; Self-assembly;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2005. IEEE-EMBS 2005. 27th Annual International Conference of the

Conference_Location

Shanghai

Print_ISBN

0-7803-8741-4

Type

conf

DOI

10.1109/IEMBS.2005.1616654

Filename

1616654