Study on the Envelope Fusion Proteins of Baculovirus

GP64 homologies, which are found in Group I NPVs, and F proteins (Fa proteins), which are utilized in Group II NPVs, are required for virion entry into the cell. And Group I NPV also encodes homologue of Fa protein, and was named Fb protein. However, Fb protein dose not appear to mediate cell fusion. To further researching these important viral envelope proteins, the expression of GP64 and Fb proteins in Group I NPVs were studied by Real-time quantitative PCR. The results indicated that Fb is not the evolution redundancy gene but an important function gene. In addition, we transfected the fragments of CbNPV Fa and ie-1 into different insect cell lines, and found that CbNPV Fa and ie-1 could not express in BmN and T.ni High Five cells but could express in Sf-9 and Spli cells.