Using Fluorescent-Based AFLP to Analyze Genetic Diversity of Yellow-Seeded Brassica Napus L.

A method for multiplex AFLP-PCR was employed to access the genetic diversity of yellow-seeded Brassica napus accessions originated from China, Czech Republic, and Poland. The AFLP-PCRs using three blended and fluorescent-labeled EcoRI+3 primers, AAC, AAG, and AC A, combing each time with one MseI+3primers CTC, CAC, CAG, CTG, CTT, and CTA, totally generated 723 peaks, of which 632 had high intension. 242 distinctly polymorphic markers among them were scored, with an average of 13.4 markers per primer pair. Based on either Dice, Jaccard, or Hamman similarity coefficients, the 31 accessions tested could be separated into Chinese, and non-Chinese group by UPGMA clustering, which corresponding well to the origins and pedigrees. Distinct geographical divergence was found among Chinese and non-Chinese yellow-seeded accessions. This AFLPs protocol were shown to be a reliable and quickly method for analyzing the genetic diversity than numeric taxonomy methods.