Novel Gene Transduction Procedures in vivo Using the Epstein-Barr Virus-based Artificial Chromosomes

Author

Mazda, Osam ; Kishida, Tsunao ; Asada, Hidetsugu ; Iida, Yasunori ; Tsunoda, Sei ; Oda, Yohei ; Shin-Ya, Masaharu ; Yoshikawa, Toshikazu ; Matsubara, Hiroaki ; Imanishi, Jiro

Author_Institution

Dept. of Microbiol., Kyoto Prefectural Univ. of Medicine, Koto

fYear

2006

Firstpage

1

Lastpage

6

Abstract

Employment of Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene and oriP sequence grants characteristics of an artificial chromosome to DNA constructs, drastically improving transduction rate as well as expression level when transfected into mammalian cells, due not only to EBNA1-dependent nuclear transport of oriP-bearing DNA molecules but also to, albeit at a lesser extent, transcriptional up-regulation mediated by oriP/EBNA1. The present study demonstrates efficient transfer of the EBV-based artificial chromosomes in vivo into cardiac muscle by means of the electroporation and sonoporation

Keywords

DNA; cellular biophysics; muscle; Epstein-Barr virus nuclear antigen 1; artificial chromosomes; cardiac muscle; electroporation; gene transduction procedure; mammalian cells; nuclear transport; oriP sequence; oriP-bearing DNA molecules; sonoporation; Biological cells; Cardiac disease; Cardiology; DNA; Immune system; In vitro; In vivo; Medical treatment; Muscles; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science, 2006 International Symposium on

Conference_Location

Nagoya

Print_ISBN

1-4244-0717-6

Electronic_ISBN

1-4244-0718-1

Type

conf

DOI

10.1109/MHS.2006.320318

Filename

4110329