Modelling the Effect of Electric Pulse on Cells and Experimental Research

Author

Xufei

Author_Institution

Sch. of Electr., Shanghai Dianji Univ., Shanghai

fYear

2008

Firstpage

880

Lastpage

883

Abstract

The effect of electric pulse to cells is simulated with a resistor-capacitor(RC) circuit model. The transmembrane voltage equation is deduced from the RC circuit model, which is proposed according to cell model with mitochondria included. The experiment settings are designed to produce pulsed electric field with the intensity peak of 2.12 MV/m within 100 ns and 1 mus, respectively. Cell death rates are assessed after electric stimulation. With the experimental parameters and cell structural, cell electric parameters from related papers, transmembrane voltages of cytomembrane and mitochondria membrane are calculated as the functions of time. Apoptosis can be induced when the mitochondria membrane is electroporated and cell will die in another way from that induced by cytomembrane electroporation. The RC circuit model and calculation correspond with the experiment result.

Keywords

RC circuits; bioelectric phenomena; biological effects of fields; biomembranes; cellular biophysics; cell death rates; cell electric parameters; cell structure; cellular biophysics; cytomembrane electroporation; electric pulse; mitochondria membrane; pulsed electric field; resistor-capacitor circuit model; transmembrane voltage equation; Biomembranes; Breakdown voltage; Cancer; Circuit simulation; Electrodes; Equations; Pulse circuits; Pulse generation; Pulse transformers; Switches;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-1747-6

Electronic_ISBN

978-1-4244-1748-3

Type

conf

DOI

10.1109/ICBBE.2008.216

Filename

4535096