Preparation and characterization of well ordered mesoporous diopside nanobiomaterial

Well ordered mesoporous diopside (OMD) nanobiomaterial was synthesized by using a sol-gel process. The in vitro bioactivity of the OMD was evaluated by investigating the apatite-forming ability in simulated body fluid (SBF), and the hemostatic activity of the OMD was determined by measuring the activated partial thromboplastin time (APTT) and prothrombin time (PT) in vitro. The results suggested that the OMD exhibited excellent in vitro bioactivity, with surface apatite formation for OMD exceeding that of non-mesoporous diopside at 7 days. Moreover, the OMD with high surface area possessed good hemostatic property because it could absorb a large number of water from the blood, which could concentrate the components of blood and reduced the clotting time. In addition, the results demonstrated the clotting time of APTT and PT could be significantly shortened because the OMD could release sufficient amounts of Ca ions, which induced an accelerated coagulation response. In conclusion, the prepared OMD had excellent bioactivity and hemostatic activity, which can not only be applied as bone repair biomaterial for bone regeneration, but also as hemostatic agent for surgery hemostasis.