All-focused image generation and 3D modeling of microscopic images of insects

We discuss a method which generates an all-focused image from a large number of microscopic images of insects. First, we describe our previously proposed select-and-merge method for all-focused image acquisition. We can get good results by using this method for two differently focused images. However, this method can not give a good result when applied to a large number of images. We propose a method which compares each images with its preceding and subsequent images using the estimation method of focused regions. We compare the results of reconstruction using the novel method and using our previously proposed method. Finally, we generate a 3D image using the intensity of the all-focused image and depths of the in-focus regions, both acquired by our proposed method