Potentiostatic deposition and detection of DNA on conductive nitrogen doped diamond-like carbon film electrode

We describe the electrochemical oxidation of deoxyribonucleic acid (DNA) at a conductive nitrogen doped diamond-like carbon (N-DLC) film electrode, and a procedure for reversible adsorption of DNA onto the N-DLC film electrode under potentiostatic control. N-DLC film electrode has well defined higher oxidation responses of guanine and adenine of denatured DNA compared to the response at a glassy carbon electrode. DNA can be deposited at the N-DLC film electrode when the electrode is positively charged and be released when the electrode is negatively charged. The N-DLC film electrode has an optimum deposition potential for DNA. The preliminary work shows that N-DLC film would be a novel electrode material for extraction and detection of DNA, which can be used for bio-micro-systems for DNA detection.