Shape-encoded particle for DNA analysis

This paper reports the use of a shape-encoded particle (SEP) as a substrate for biochemical probe (such as DNA or protein) attachment and identification. Its application involves large-scale genomic/proteomic analysis of multiple data based on shape-recognizable microparticles. Many samples from different sources (e.g. DNA/RNA from multiple subjects) can be assayed in parallel on the microparticles with known probes immobilized onto the surface in a way similar to DNA analysis. The information obtained (i.e. hybridization data) can then be sorted out and re-associated back to the specific probe based on encoded shape on the microparticles. Our goal is to provide a simple tool to greatly simplify the DNA microarray procedure, and allow more flexibility and better quality control in experimental design. Compared to other barcoding techniques, SEPs are easy to fabricate, low cost, unlimited supply of distinguishable particles, and non-interfering with the reporter signals.