Surfactant therapy after electroporation mediated muscle injury in vivo

Author

Hannig, Jurgen ; Block, Thomas A. ; Matthews, Kenneth L., II ; Chen, Chin-Tu ; Lee, Raphael C.

Author_Institution

Dept. of Surg., Chicago Univ., IL, USA

Volume

3

fYear

2000

fDate

2000

Abstract

High voltage electrical shock can produce extensive muscle and nerve tissue damage due to poration of cell membranes by electric field gradients in addition to thermal denaturation of biomolecules. The authors studied the therapeutic membrane sealing effect of the surfactant Poloxamer 188 on muscle tissue damage in vivo following non-thermal electrical injury in their rat hind limb model (DC-pulses, 150 V/cm) by radiotracer imaging. Poloxamer 188 surfactant injections (50 mg/kg) showed a significant decrease in radiotracer retention compared to control injections post-shock. The results indicate the importance of membrane permeabilization and the therapeutic potential of surfactant sealing in electrical shock victims

Keywords

bioelectric phenomena; biomembrane transport; biothermics; muscle; patient treatment; surfactants; biomolecules thermal denaturation; electric field gradient; electrical shock victims; electroporation mediated muscle injury; membrane permeabilization; muscle tissue damage; nerve tissue damage; nonthermal electrical injury; surfactant Poloxamer 188; surfactant sealing; surfactant therapy; therapeutic potential; Biomembranes; Cells (biology); Electric shock; Injuries; Medical treatment; Molecular biophysics; Muscles; Nerve tissues; Tissue damage; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2000. Proceedings of the 22nd Annual International Conference of the IEEE

Conference_Location

Chicago, IL

ISSN

1094-687X

Print_ISBN

0-7803-6465-1

Type

conf

DOI

10.1109/IEMBS.2000.901138

Filename

901138