DocumentCode
1753182
Title
An AFM Method for in situ Probing Membrane Proteins under Physiological Condition
Author
Li, Guangyong ; Xi, Ning ; Chan, Ho-Yin ; Wang, Donna H.
Author_Institution
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, Email: liguangyegr.msu.edu
Volume
1
fYear
2006
fDate
17-20 June 2006
Firstpage
181
Lastpage
184
Abstract
Although membrane proteins are the main drug targets, the study of cell membrane proteins in situ at the molecular level is difficult due to the current technical limitations. The recent development of atomic force microscopy (AFM) opens a new avenue to study the functionalities of cell membrane proteins. In this paper, techniques involving the use of AFM to probe biomolecules are introduced. The techniques for characterizing specific single receptor using the functionalized AFM tip is discussed. An example of probing angiotensin II type 1 (AT1) receptor by functionalized AFM tip is also given in this paper.
Keywords
Atomic force microscopy; Biomembranes; Cells (biology); High-resolution imaging; Interference; Molecular biophysics; Polymers; Probes; Protein engineering; Surface topography;
fLanguage
English
Publisher
ieee
Conference_Titel
Nanotechnology, 2006. IEEE-NANO 2006. Sixth IEEE Conference on
Print_ISBN
1-4244-0077-5
Type
conf
DOI
10.1109/NANO.2006.247602
Filename
1717052
Link To Document