A Novel Lensless Miniature Contact Imaging System for Monitoring Calcium Changes in Live Neurons

Author

Mudraboyina, Anil Kumar ; Blockstein, Lior ; Luk, Collin C. ; Syed, Naweed I. ; Yadid-Pecht, O.

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Calgary, Calgary, AB, Canada

Volume

6

Issue

1

fYear

2014

fDate

Feb. 2014

Firstpage

1

Lastpage

15

Abstract

Here, we report on the design, fabrication, and verification of a novel CMOS-imager-based contact imaging system. We acquired fluorescent images from live neurons by monitoring calcium changes with Fura-2 dye. Our current device consists of a removable absorption filter interfaced with a CMOS imaging sensor and an external DG-4 lamp for excitation. Fura-2 loaded Lymnaea stagnalis neurons were stimulated with dual excitation wavelengths of 340 and 380 nm; our image sensor detected 510-nm emission. We show that our system is capable of detecting intracellular calcium changes in Fura-2 loaded neurons. Further, this sensor also enabled viewing of multiple neurons over a large surface area simultaneously, an option that is not readily available in conventional light microscopy.

Keywords

CMOS image sensors; biomedical optical imaging; dyes; fluorescence; neurophysiology; optical microscopy; CMOS imaging sensor; CMOS-imager-based contact imaging system; DG-4 lamp; Fura-2 dye; Fura-2 loaded Lymnaea stagnalis neurons; absorption filter; fluorescent imaging; large surface area; lensless miniature contact imaging system; light microscopy; live neurons; wavelength 340 nm; wavelength 380 nm; wavelength 510 nm; Arrays; Band-pass filters; Calcium; Fluorescence; Glass; Imaging; Optical filters; Contact Imaging; Fura-2 AM; fluorescence; fluorescent contact imaging;

fLanguage

English

Journal_Title

Photonics Journal, IEEE

Publisher

ieee

ISSN

1943-0655

Type

jour

DOI

10.1109/JPHOT.2014.2304554

Filename

6731561