Non-contact measurement of oxygen consumption rate of single oocyte using stripe-shaped fluorescence sensor on microfluidic chip

Author

Kito, Masanobu ; Maruyama, Hisataka ; Arai, Fumihito

Author_Institution

Dept. of Mech. Sci. of Eng., Nagoya Univ., Nagoya, Japan

fYear

2014

fDate

10-12 Nov. 2014

Firstpage

1

Lastpage

2

Abstract

We developed measurement method of oxygen consumption rate (OCR) of individual oocyte using stripe-shape fluorescence sensor on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate (PEG-DA) and Tris (2,2´-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The sensor was photo-patterned to stripe-shape in the polydimethylsiloxane (PDMS). Oxygen around the oocyte can be spherically diffused because of high oxygen permeability of PDMS and the stripe pattern of sensor. This sensor achieves two-dimensional measurement of OCR by image processing. We demonstrated non-contact measurement of OCR of single oocyte on a microfluidic chip.

Keywords

bioMEMS; biochemistry; biomedical optical imaging; cellular biophysics; electrochemical sensors; fluorescence; medical image processing; microfluidics; microsensors; optical sensors; polymers; image processing; microfluidic chip; noncontact measurement; oxygen consumption rate; oxygen permeability; photopatterning; polydimethylsiloxane; polyethylene glycol diacrylate; single oocyte; spherical diffusion; stripe-shape fluorescence sensor; tris(2,2´-bipyridyl) dichloro ruthenium (II) hexahydrate; two-dimensional measurement; Biomedical measurement; Fluorescence; Mice; Microfluidics; Optical character recognition software; Oxygen; Semiconductor device measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-6678-3

Type

conf

DOI

10.1109/MHS.2014.7006137

Filename

7006137