Cell pairing on a microwell array electrode by positive dielectrophoresis

Author

Yasukawa, Tomoyuki ; Yoshimura, Yuki ; Mizutani, Fumikazu

Author_Institution

Grad. Sch. of Mater. Sci., Univ. of Hyogo, Ako, Japan

fYear

2014

fDate

10-12 Nov. 2014

Firstpage

1

Lastpage

2

Abstract

We present single-cell pairing of different types of cells with a rapid manipulation based on positive dielectrophoresis (p-DEP). The DEP device for the manipulation of cells consisted of a microfluidic channel with an upper indium tin oxide (ITO) electrode and a lower ITO electrode with 10,000 (100 × 100) microwells. The width (14 μm) and depth (25 μm) of the individual microwells restricted the size to two vertically aligned cells. Cells stained in blue and stained in green were continuously trapped in the microwells. Cells were paired within only 1 min and a pairing efficiency of 53% was achieved.

Keywords

bioMEMS; biological techniques; cellular biophysics; electrochemical electrodes; electrophoresis; indium compounds; microfluidics; DEP device; ITO; ITO electrode; microfluidic channel; microwell array electrode; positive dielectrophoresis; single-cell pairing efficiency; upper indium tin oxide electrode; Arrays; Dielectrophoresis; Electrodes; Fluorescence; Indium tin oxide; Microfluidics; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-6678-3

Type

conf

DOI

10.1109/MHS.2014.7006159

Filename

7006159