Electrodeposition of alginate hydrogel for spatially selective entrapment of biological cells

Author

Zeyang Liu ; Takeuchi, Masaru ; Nakajima, Masahiro ; Fukuda, Toshio ; Hasegawa, Yohei ; Qiang Huang

Author_Institution

Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan

fYear

2014

fDate

10-12 Nov. 2014

Firstpage

1

Lastpage

2

Abstract

In this paper, an electrodeposition method was applied to construct cell patterning structures by an alginate hydrogel. The liver cells (RLC-18) were trapped selectively within alginate structures on the micro-patterning electrodes, which were fabricated by a photo-lithography process in circular, ring, and triangle structures. The living conditions of RLC-18 cells were confirmed until 3 days. These results indicate that biological cells were entrapped inside arbitrary shape hydrogel structures in living conditions. The fabricated 2D biological structures are important to be applied for 3D assembly of biological cells for tissue engineering applications.

Keywords

biomedical materials; cellular biophysics; electrodeposition; hydrogels; liver; photolithography; tissue engineering; 2D biological structures; 3D assembly; RLC-18 cells; alginate hydrogel; alginate structures; arbitrary shape hydrogel structures; biological cells; cell patterning structures; circular structures; electrodeposition method; liver cells; living conditions; micropatterning electrodes; photolithography process; ring structures; spatially selective entrapment; tissue engineering applications; triangle structures; Assembly; Computer architecture; Educational institutions; Microprocessors; Shape; Three-dimensional displays; Tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2014 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4799-6678-3

Type

conf

DOI

10.1109/MHS.2014.7006162

Filename

7006162