The exposure method for digital microscopic image

To compensate for the uneven brightness of image caused by the various light sources of optical microscopy, this paper proposes a exposure method for digital microscopic image. Firstly, for uneven illumination of characteristics of the optical microscopic digital imaging systems, it draws gain curve according to no slice of pure background brightness of the image and gains brightness gain equation in the case of the exposure situation by the histogram and the brightness distribution by brightness curve. Then it calculates the brightness adjustment factor when the brightness of no slice of pure background image is adjusted even. Under the same conditions, it uses the brightness adjustment factor on the corresponding pixel brightness values of the slice image to make the image brightness uniformity. Finally it uses the above method to test. The results show that this method can precisely realize to adjust the brightness of the optical microscopic digital imaging system.