Quantitative analysis of immunofluorescent retinal images

Author

Byun, Jiyun ; Vu, Nhat ; Sumengen, Baris ; Manjunath, B.S.

Author_Institution

Center for Bio-image Informatics, California Univ., Santa Barbara, CA

fYear

2006

fDate

6-9 April 2006

Firstpage

1268

Lastpage

1271

Abstract

We present a novel method to quantitatively analyze confocal microscope images of retinas. We automatically detect nuclei within the outer nuclear layer (ONL) in a retinal image. Based on nuclei detection results, we also automatically measure the thickness of the ONL and the local cell density within the ONL. These measurements provide the first thorough quantitative analysis of retinal images. Our results not only verify previous conclusions about retinal restructuring during detachment, but also provide biologists with significant information about the regional responses in the ONL

Keywords

biomedical optical imaging; cellular biophysics; eye; medical image processing; automatic nuclei detection; confocal microscope images; local cell density; outer nuclear layer thickness; quantitative immunofluorescent retinal image analysis; retinal restructuring; Biological tissues; Cells (biology); Density measurement; Image analysis; Image segmentation; Microscopy; Nuclear measurements; Photoreceptors; Retina; Thickness measurement;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: Nano to Macro, 2006. 3rd IEEE International Symposium on

Conference_Location

Arlington, VA

Print_ISBN

0-7803-9576-X

Type

conf

DOI

10.1109/ISBI.2006.1625156

Filename

1625156