Multiple-compartment chip for parallel recordings of epileptic activity from organotypic cultures

Author

Berdichevsky, Y. ; Staley, K.J.

Author_Institution

Med. Sch., Massachusetts Gen. Hosp., Dept. of Neurology, Harvard Univ., Boston, MA, USA

fYear

2011

fDate

April 27 2011-May 1 2011

Firstpage

572

Lastpage

575

Abstract

Organotypic hippocampal slice culture is an in vitro model of post-traumatic epilepsy. To increase experimental throughput for development of anti-epileptic drugs, we designed a system for parallel recordings of neural activity from several cultures. We recorded spontaneous electrical activity from four organotypic cultures on the same recording chip for more than seven weeks. We confirmed that hippocampal cultures develop spontaneous epileptiform activity. Cultures exhibited a quiet latent period followed by interictal and ictal epileptiform activity.

Keywords

bioMEMS; bioelectric phenomena; biomedical electrodes; biomedical measurement; brain; cellular biophysics; diseases; neurophysiology; antiepileptic drug development; epileptic activity parallel recordings; ictal epileptiform activity; interictal epileptiform activity; multiple compartment chip; neural activity parallel recordings; organotypic cultures; organotypic hippocampal slice culture; post traumatic epilepsy in vitro model; recording chip; spontaneous electrical activity; spontaneous epileptiform activity; Drugs; Electrodes; Epilepsy; In vitro; Injuries; Neurons; Noise;

fLanguage

English

Publisher

ieee

Conference_Titel

Neural Engineering (NER), 2011 5th International IEEE/EMBS Conference on

Conference_Location

Cancun

ISSN

1948-3546

Print_ISBN

978-1-4244-4140-2

Type

conf

DOI

10.1109/NER.2011.5910613

Filename

5910613