Surface-bound biomembrane assemblies

Author

Smith, H. Gilbert ; Li, Jianguo ; Downer, Nancy W. ; DeLuca, Leslie W.

Author_Institution

EG&G Mason Res. Inf., Worcester, MA, USA

fYear

1989

fDate

9-12 Nov 1989

Firstpage

1329

Abstract

A modified detergent dialysis technique was used to assemble membrane structures containing the integral membrane receptor protein, rhodopsin, on alkylsilanated surface. Lipid and protein compositions and electrochemical capacitance measurements were consistent with a surface-bound bilayer structure incorporating the receptor protein. Rhodopsin incorporated into the surface-bound structures retained its native absorption spectra and its ability to activate enzymatic processes characteristic of visual excitation

Keywords

biomembranes; molecular biophysics; proteins; alkylsilanated surface; electrochemical capacitance measurements; enzymatic processes; integral membrane receptor protein; lipid compositions; modified detergent dialysis technique; native absorption spectra; protein compositions; receptor protein; rhodopsin; surface bound biomembrane assemblies; surface-bound bilayer structure; visual excitation; Absorption; Area measurement; Assembly; Biomembranes; Capacitance measurement; Composite materials; Glass; Lipidomics; Photoreceptors; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1989. Images of the Twenty-First Century., Proceedings of the Annual International Conference of the IEEE Engineering in

Conference_Location

Seattle, WA

Type

conf

DOI

10.1109/IEMBS.1989.96222

Filename

96222