Cells tracking in a live zebrafish embryo

Author

Melani, C. ; Peyrieras, N. ; Mikula, K. ; Zanella, C. ; Campana, M. ; Rizzi, B. ; Veronesi, F. ; Sarti, A. ; Lombardot, B. ; Bourgine, P.

Author_Institution

Univ. di Bologna, Bologna

fYear

2007

fDate

22-26 Aug. 2007

Firstpage

1631

Lastpage

1634

Abstract

We designed a set of procedures for achieving the tracking of cell nuclei and the identification of cell divisions in live zebrafish embryos using 3D+time images acquired by confocal laser scanning microscopy (CLSM). Our strategy includes image signal enhancement with feature preserving denoising algorithm, automated identification of the nuclei position, extraction of the optical flow from 3D images sequences and tracking of nuclei.

Keywords

biomedical optical imaging; cellular biophysics; image denoising; image enhancement; image sequences; laser applications in medicine; medical image processing; optical microscopy; optical tracking; 3D images sequences; 3D-time images; CLSM; automated identification; cell division identification; cell nuclei position; cell nuclei tracking; confocal laser scanning microscopy; feature preserving denoising algorithm; image signal enhancement; live zebrafish embryo; optical flow extraction; Biomembranes; Embryo; Image reconstruction; In vivo; Microscopy; Noise reduction; Optical design; Optical imaging; Proteins; Signal processing; Algorithms; Animals; Artificial Intelligence; Embryo, Nonmammalian; Image Enhancement; Image Interpretation, Computer-Assisted; Imaging, Three-Dimensional; Microscopy, Confocal; Microscopy, Fluorescence; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity; Zebrafish;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE

Conference_Location

Lyon

ISSN

1557-170X

Print_ISBN

978-1-4244-0787-3

Type

conf

DOI

10.1109/IEMBS.2007.4352619

Filename

4352619