A cell culture system with better spatial and time resolution

Author

Ou, Yu-Cheng ; Hsu, Chih-Wen ; Yang, Lung-Jieh ; Han, Hsieh-Cheng ; Chen, Chien-Yuan

Author_Institution

Dept. of Mech. & Electro-Mech. Eng., Tamkang Univ., Tamsui, Taiwan

fYear

2009

fDate

18-21 Oct. 2009

Firstpage

89

Lastpage

93

Abstract

A new cell culture system is demonstrated in this paper. This system includes a culture incubator with a compact optical monitoring module and an electro-chemical impedance sensor (ECIS) chip with gelatin micro patterns attracting living cells selectively. We provided a platform with a shorter observation interval of 4 hours and a finer line width of electrodes of sensor chips than the prior art in this work. The in-situ impedance measurement on HeLa cells was performed and shown the better spatial and time resolution.

Keywords

bioelectric phenomena; biological techniques; biosensors; cellular biophysics; electric impedance; electric impedance measurement; electrochemical sensors; gelatin; lab-on-a-chip; microsensors; molecular biophysics; optical sensors; HeLa cells; cell culture system; compact optical monitoring module; electrochemical impedance sensor chip; gelatin micropatterns; in-situ impedance measurement; spatial resolution; time resolution; Biomedical monitoring; Biomedical optical imaging; Integrated optics; Monitoring; Optical device fabrication; Optical imaging; Optical sensors; cell culture; gelatin; glutaraldehyde; impedance sensing; in-situ monitoring;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Molecular Medicine and Engineering (NANOMED), 2009 IEEE International Conference on

Conference_Location

Tainan

Print_ISBN

978-1-4244-5528-7

Type

conf

DOI

10.1109/NANOMED.2009.5559113

Filename

5559113