Microfabrication of asymmetric planar electrodes for long-term glucose monitoring

In the present study, we proposed a design of asymmetric planar electrodes for long-term monitoring of glucose level at body temperature. The asymmetric planar electrodes were fabricated by thin film technology on glass and cut as chip sensor. The glass-based chip sensor was further immobilized with glucose oxidase for glucose measuring. To investigate the stability of the glass-based chip sensor, we performed a long-term study and compared with a commercial polymer sensor. All of the experiments were carried out in phosphate buffer solution at 37°C. The result indicated that the glass-based chip senor was more stable in background signal than a commercial polymer sensor during a 18 days of study. The chip senor immobilized with glucose oxidase showed fast response time and broad detection range for glucose measurement. The present study demonstrated that our design of asymmetric planar electrodes was suitable for developing a glucose sensor with long-term stability.