Electric Impedance Assisted Micropipette Aspiration

Author

Kovanen, K. ; Lukkari, M. ; Purmonen, S. ; Ylikomi, T. ; Viitanen, J. ; Kallio, P.J.

Author_Institution

Tampere Univ. of Technol., Tampere

fYear

2007

fDate

22-26 Aug. 2007

Firstpage

5822

Lastpage

5825

Abstract

Micropipette aspiration is a technique to selectively isolate cells from cell cultures using suction pressure. Cells can selectively be isolated one by one from neighboring cells into the micropipette. This paper presents a novel micropipette aspiration system assisted by an impedance measurement system. Furthermore, a method to reduce the adhesion force at a single cell level for a gentler detachment of the cell from a cultivation surface and surrounding cell connections is proposed.

Keywords

adhesion; bioelectric phenomena; biomechanics; biomedical measurement; cellular biophysics; electric impedance measurement; adhesion force; cell connection; cell culture; cell deformation; cell isolation; cultivation surface; electric impedance measurement system; micropipette aspiration system; single cell level; suction pressure; Adhesives; Automatic control; Automation; Control systems; Impedance measurement; Magnetic levitation; Mechanical factors; Medical control systems; Microinjection; Micromanipulators; Micropipette aspiration; adherent cells; impedance measurement; microinjection; micromanipulation; Cell Physiological Phenomena; Cell Separation; Cells, Cultured; Electric Impedance; Electrochemistry; Humans; Micromanipulation; Suction;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE

Conference_Location

Lyon

ISSN

1557-170X

Print_ISBN

978-1-4244-0787-3

Type

conf

DOI

10.1109/IEMBS.2007.4353671

Filename

4353671