Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish

Author

Matsumoto, Tad ; Aoyagi, Shigehisa

Author_Institution

Dept. of Biomater., Okayama Univ., Okayama, Japan

fYear

2012

fDate

4-7 Nov. 2012

Firstpage

175

Lastpage

177

Abstract

The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O₂ plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.

Keywords

biochemistry; biological tissues; biomedical materials; cellular biophysics; microfabrication; molecular biophysics; polymers; proteins; tissue engineering; O₂ plasma treatment; chemical modification; extracted submandibular gland tissue; fibronectin coating; mesenchymal cells; microfabricated surface; micropatterned tissue culture dish; micropillar structure; physical modification; polydimethylsiloxane tissue culture substrate; surface affinity; tissue development;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2012 International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4673-4811-9

Type

conf

DOI

10.1109/MHS.2012.6492441

Filename

6492441