One core-five sheaths coaxial flow formation using multilayer stacked flow focusing structure

This research presents efficient coaxial sheath flow formation using a three-dimensional PDMS device. In the device, one core and five sheaths are simply formed with low diffusion between different samples. Stacking and alignment of six PDMS layers of same structure allowed for fabrication of the proposed 3D device. Only one point alignment of center channel for the sheath provides free from misalignment that was a critical problem of multi-stacked PDMS device. Furthermore, the number of samples is infinitely expandable by increase in the number of stacking layers. The coaxial sheath flow is useful for biological fiber formation which requires multilayer of different materials such as artificial blood vessels or muscles.