Cell-laden hinged microplates for measuring the contractile forces of cardiomyocytes

Author

Matsumoto, Hiroaki ; Yoshida, Shotaro ; Morimoto, Yuya ; Mori, Nobuhito ; Serien, Daniela ; Takeuchi, Shoji

Author_Institution

Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan

fYear

2015

fDate

18-22 Jan. 2015

Firstpage

589

Lastpage

592

Abstract

We report a method to measure contractile forces of cardiomyocytes at cellular level using microplates; pairs of single-cell sized plates are connected by a flexible hinge at the center. Cardiomyocytes repeatedly contract and relax, and thereby fold and stretch the microplates at the flexible hinge. By measuring the change of the bending angle in folded microplates, we can estimate contractile forces of cardiomyocytes. We believe that this method is a useful tool to study the biomechanics of cardiomyocytes.

Keywords

angular measurement; bending; biomechanics; biomedical equipment; biomedical measurement; cardiology; cellular biophysics; force measurement; hinges; muscle; cardiomyocyte biomechanics; cardiomyocyte contractile force estimation; cardiomyocyte contractile force measurement; cardiomyocyte contraction; cardiomyocyte relaxation; cell-laden hinged microplate; cellular level measurement; flexible hinge; folded microplate bending angle change measurement; microplate folding; microplate stretching; single-cell sized plate pair; Atmospheric measurements; Fabrication; Fasteners; Force; Force measurement; Heart; Physiology;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on

Conference_Location

Estoril

Type

conf

DOI

10.1109/MEMSYS.2015.7051024

Filename

7051024