Minimally invasive micromanipulation of microbe by laser trapped micro tools

Reports separation of the target microbe by the laser trapped micro tools for minimum invasion of laser irradiation. The size of a micro tool (MT) is around micrometer. The micro tools are manipulated by the focused lasers under the microscope to manipulate the target microbe. We propose a pinpoint injection method of micro tools at the desired location in the micro chamber, which is filled with liquid. First, we classified the injection method of the micro tools in 4 categories. We employed a new method to install the micro tools inside the micro chamber. We developed a micro tool holding chip to install the micro tools. The micro tools were injected in the micro chamber, and were manipulated successfully by the laser scanning micromanipulator to separate the target microbe. The proposed method is useful for the pinpoint injection of MTs and separation by the indirect micromanipulation.