Steering wheel photonic crystal fiber for human IgG detection

Author

Rabah, Jad ; Mansaray, Alpha ; Wynne, Rosalind ; Duran, Metin

Author_Institution

Electr. & Comput. Eng. Dept., Villanova Univ., Villanova, PA, USA

fYear

2014

fDate

2-5 Nov. 2014

Firstpage

1403

Lastpage

1406

Abstract

A solid-core photonic crystal fiber (SC-PCF) sensor with a microstructured all-silica steering wheel lattice containing a 3μm diameter core is used to identify dye-free monoclonal antibodies (mAbs) in very small volumes (i.e. <; 0.001 μL) that are useful in detecting the presence of immunobased assays and susceptible to secondary antigens specific to the detection application. A 0.8 m length of SC-PCF was used with a 3.74 nL sample and a broadband LED in the 1300-1700 nm range. This experiment uniquely investigates the spectral characteristics of purified Human immunoglobulin class G (IgG) (format-UNLB) in aqueous media. The absorption characteristics associated with these mAbs were measured using a standard optical spectrum analyzer.

Keywords

biomedical equipment; fibre optic sensors; holey fibres; light emitting diodes; molecular biophysics; photonic crystals; proteins; SC-PCF; absorption characteristics; aqueous media; broadband LED; dye-free monoclonal antibodies; format-UNLB; human IgG detection; immunobased assays; microstructured silica steering wheel lattice; optical spectrum analyzer; purified human immunoglobulin class G; secondary antigens; solid-core photonic crystal fiber sensor; spectral characteristics; steering wheel photonic crystal fiber; Absorption; Biomedical optical imaging; Immune system; Optical fiber sensors; Optical fibers;

fLanguage

English

Publisher

ieee

Conference_Titel

SENSORS, 2014 IEEE

Conference_Location

Valencia

Type

conf

DOI

10.1109/ICSENS.2014.6985275

Filename

6985275