The Research of Biosensor Building on F0F1-ATPase

A biosensor building on F₀F₁-ATPase for clenbuterol detection is constructed. The energy for ATP synthesis is provided from downhill proton transport along the proton gradient across membranes. This potential is formed by the electron transfer chains of respiration. The fluorescence probe F1300, which was sensitive to pH changes, was labeled into inner chromatophores to indicate the proton-flux during ATP synthesis. It´s found that different loads onto the beta-subunit of chromatophores could affect its synthesis activity with different level. According to this principle, a biosensor was constructed based on ATPase to detect clenbuterol and the detecting mechanism depended on proton-flux driven by rotary catalytic ATP synthesis. The experiment results showed that the precision of clenbuterol detection by the biosensor could reach 10^-12 g/L and the new biosensor might prove to be a useful nano-device for super-sensitive detection.