Functional connections between avian and mammalian neurons

The genetical controls are critical for constructing specific circuit in a brain, however, also a distant relationship likes a mammal and a bird, the fundamental structure and functions of neurons are preserved. Using Ca²⁺ imaging of a mixed culture of rat and chick neurons, we elucidated whether the functional connections are formed between neurons from relatively distant species in this study. Periodic and synchronized Ca²⁺ transients were often observed. Some synchronizations were not strict, suggesting that the bursting activity was loosely transmitted each other. A coculture of rat and chick neuronal cells was possible and some of neurons from distinct species made functional connections, which can be utilized to complementation of damaged neuronal circuit. The mixed circuit of cultured neurons from distinct species is the interesting testing platform for elucidating conditions required for a synaptogenesis and activity of a circuit of neurons from mixed species.