The Detection of Bacteria on Microarrays Using Up-Converting Phosphor Nanoparticles as Fluorescent Labels

Author

Guo, Yonggang ; Deng, Wei ; Guo, Min ; Chen, Depu ; Cheng, Jing

Author_Institution

Med. Syst. Biol. Res. Center, Tsinghua Univ., Beijing

fYear

2006

fDate

18-21 Jan. 2006

Firstpage

550

Lastpage

555

Abstract

Nanoparticles containing the infrared-to-visible up-converting phosphors (UCP) ytterbium and erbium and co-doped with sodium yttrium fluoride were synthesized with diameters ranging from 30 nm to 50 nm. These high luminescence efficient UCP beads were employed as detection labels and applied in microarrays for the detection of specific nucleic acid hybrids to identify bacteria for the first time. The low cost of UCP nanoparticles and their detection equipment may facilitate their broad application in areas requiring large numbers of assays such as clinical diagnostic and environmental monitoring

Keywords

arrays; bioMEMS; biosensors; doping; erbium; fluorescence; microorganisms; nanoparticles; phosphors; sodium compounds; ytterbium; 30 to 50 nm; NaYF₄:Yb,Er; bacteria detection; detection labels; fluorescent labels; infrared-visible UCP; luminescence efficiency; microarrays; nucleic acid hybrid detection; up-converting phosphor nanoparticles; ytterbium-erbium codoped sodium yttrium fluoride; Costs; Erbium; Fluorescence; Luminescence; Microorganisms; Monitoring; Nanoparticles; Phosphors; Ytterbium; Yttrium; UCP; microarray; nanoparticals; streptavidin;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems, 2006. NEMS '06. 1st IEEE International Conference on

Conference_Location

Zhuhai

Print_ISBN

1-4244-0139-9

Electronic_ISBN

1-4244-0140-2

Type

conf

DOI

10.1109/NEMS.2006.334838

Filename

4135015